Effect of Environmental Concentration of Carbamazepine on the Behaviour and Gene Expression of Laboratory Rats.

Carbamazepine (CBZ), an effective drug for epilepsy and other neurological diseases, and its metabolites are one of the most frequently detected substances in the aquatic environment. Although these are doses of very low concentrations, chronic exposure to them can affect the physiological processes of living organisms. This experiment may clarify if carbamazepine, under an environmental and a therapeutic concentration, can affect the behaviour of higher vertebrates, especially mammals, and gene expressions of Ugt1a6 and Ugt1a7 in the brain compared to the control group without exposure to CBZ. Three groups of thirteen rats were randomly formed, and each group was treated either with carbamazepine 12 mg/kg (therapeutic), carbamazepine 0.1 mg/kg (environmental), or by 10% DMSO solution (control). The memory, anxiety, and social behaviour of the rats were assessed by the test Elevated Plus Maze, the novel object recognition test, and the social chamber paradigm. After testing, they were euthanised and brain tissue samples were collected and analysed for mRNA expression of Ugt1a6 and Ugt1a7 genes. The tests did not show significant differences in the behaviour of the rats between the groups. However, there were significant changes at the gene expression level of Ugt1a7.

Genetic Parameters of Performance and Conformation Traits of 3-Year-Old Warmblood Sport Horses in the Czech Republic.

The aim of this study was to estimate the genetic parameters of a one-day performance test together with the linear type traits of 3-year-old warmblood horses. The study of genetic parameters was based on 5958 tested horses in the period 1998-2021. A total of 22 traits of linear description, three quantitatively measured traits, and one summary mark from the performance test were tested. The model equation included the fixed effect of gender and combination effects of classifier-year of evaluation-place. A single-trait animal model was used for the estimation of heritability and genetic variance, while the two-trait animal model was applied for the estimation of variance and covariance between all traits. The heritability of the overall score of the performance test was 0.25. The range for heritability was between 0.04 and 0.33 for the linear type traits and between 0.46 and 0.57 for the quantitatively measured traits. Genetic correlations were between -0.47 and 0.92. The estimated genetic parameters suggest that the results from the performance test can be incorporated into genetic evaluation in the Czech Republic.

How Parents Perceive the Potential Risk of a Child-Dog Interaction.

Dog attacks on children are a widespread problem, which can occur when parents fail to realise a potentially dangerous interaction between a dog and a child. The aim of the study was to evaluate the ability of parents to identify dangerous situations from several everyday child-dog interactions and to determine whether the participants connected these situations to a particular breed of dog. Five sets of photographs depicting potentially dangerous interactions from everyday situations between children and three dogs (one of each breed) were presented via an online survey to parents of children no more than 6 years old. Data from 207 respondents were analysed using proc GLIMMIX in SAS program, version 9.3. The probability of risk assessment varied according to dog breed (p < 0.001) as well as to the depicted situation (p < 0.001). Results indicated that Labrador Retriever was considered the least likely of the three dogs to be involved in a dangerous dog-child interaction (with 49% predicting a dangerous interaction), followed by Parson Russell Terrier (63.2%) and American Pit Bull Terrier (65%). Participants considered one particular dog-child interaction named 'touching a bowl' a dangerous interaction at a high rate (77.9%) when compared with the other presented situations, which were assessed as dangerous at rates of 48.4% to 56.5%. The breed of dog seems to be an influential factor when assessing a potentially dangerous outcome from a dog-child interaction. Contrary to our hypothesis, interactions involving the small dog (Russell Terrier) were rated more critically, similarly to those of the Pit Bull Terrier. These results suggest that even popular family dog breeds, such as Labrador Retrievers, should be treated with more caution.

Ability of dog owners to identify their dogs by smell.

Several studies report that olfactory cues play an important role in human life; humans are essentially able to recognize other family members and friends by their odors. Moreover, recent studies report that humans are also able to identify odors of non-conspecifics. The aim of this study was to determine whether dog owners are able to identify their dogs by smell and distinguish the odor of their own dogs from those of other dogs. A total of 53 dog owners (40 females and 13 males of different ages) volunteered to take part in this study. A number of the participants (17) owned 2 dogs; these owners took part in the study twice (i.e., working with only one dog at a time). Sterile gauze pads were used to collect odor samples from the dogs. Each pad was placed in its own sterile glass jar (750 ml) with a twist off lid until the experiment commenced. Participants were asked to identify their own dog´s odor from a line-up of 6 glass containers. This experiment demonstrated that dog owners are capable of identifying their dogs by smell on a significant level. Results of this study additionally suggested that male owners outperformed their female counterparts in the identification process. Moreover, dog owners whose dogs were housed outside had a higher success rate in identification than did participants who kept their dogs indoors with them. The dog owners found it easier to identify dogs that had been neutered, fed dry dog food and bathed less frequently. In general, younger dog owners tended to have more success when attempting to identify their dogs than did their older counterparts.

Czechoslovakian Wolfdog Genomic Divergence from Its Ancestors Canis lupus, German Shepherd Dog, and Different Sheepdogs of European Origin.

This study focused on the genomic differences between the Czechoslovakian wolfdog (CWD) and its ancestors, the Grey wolf (GW) and German Shepherd dog. The Saarloos wolfdog and Belgian Shepherd dog were also included to study the level of GW genetics retained in the genome of domesticated breeds. The dataset consisted of 131 animals and 143,593 single nucleotide polymorphisms (SNPs). The effects of demographic history on the overall genome structure were determined by screening the distribution of the homozygous segments. The genetic variance distributed within and between groups was quantified by genetic distances, the FST index, and discriminant analysis of principal components. Fine-scale population stratification due to specific morphological and behavioural traits was assessed by principal component and factorial analyses. In the CWD, a demographic history effect was manifested mainly in a high genome-wide proportion of short homozygous segments corresponding to a historical load of inbreeding derived from founders. The observed proportion of long homozygous segments indicated that the inbreeding events shaped the CWD genome relatively recently compared to other groups. Even if there was a significant increase in genetic similarity among wolf-like breeds, they were genetically separated from each other. Moreover, this study showed that the CWD genome carries private alleles that are not found in either wolves or other dog breeds analysed in this study.